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image-based analysis of intra-cellular trafficking

18 mois
Date de début: 
Vendredi, Juin 1, 2018
Date limite de candidature: 
Dimanche, Avril 1, 2018

Context :

Lattice Light Sheet Microscopy (LLS-M) represents at present the novel generation of 3D fluorescence microscopes dedicated to single cell analysis, generating extraordinarily sharp, 3D images and videos. However, the usual conventional image processing algorithms developed for fluorescence microscopy are likely to fail to process the deluge of voxels generated by LLS-M instruments.

The DALLISH project, associating INRIA/SERPICO-BEAGLE, INRA/MaIAGE, and Curie Institute, aims at improving the core of 3D image processing and quantification methods to face this computational challenge. The proposed methods will be used to decipher mechanisms involved in protein transport observed in LLS-M experiments.

Understanding the molecular and cellular mechanisms underlying membrane traffic pathways is central in fundamental cell biology. Here, we propose to combine LLS-M, quantitative bioimaging, modelling and cell biology to assess the functional properties of molecular complexes and elucidate the role of key molecules involved in the endocytosis and exocytosis pathways.

Objective :

Build a spatio-temporal map of intracellular traffic of several molecular species from a set of partial observations (3D two-channels movies) revealing only one or two molecular species at a time.

Trajectories of molecular cluster will be provided by the SERPICO team. The aim will be to analyse them and in particular to identify and characterise the dependencies between molecular species and the possible changes in behaviour associated with them. This analysis could be performed through classification approaches but also modelling guided by biological a priori (e.g. PDMP models). The long-term objective is to label trajectories over time according to possible interactions with other molecular species.

Skills :

The successful candidate should

  • Hold a PhD in biophysics or image based modelling

  • Have advanced programming skills in C++, Java or Python

  • Have a first experience in effective collaboration with researchers from diverse backgrounds including biology will be appreciated

Date de validité: 
Jeudi, Mai 17, 2018
Trubuil Alain, alain.trubuil@inra.fr
Labarthe Simon, simon.labarthe@inra.fr
Laroche Béatrice, beatrice.laroche@inra.fr

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Offre_d_emploi | by Dr. Radut