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19 janvier 2015

Français
Heure et lieu: 
11h, salle de réunion, bâtiment 210
Nom intervenant: 
Bogdan Mirauta
Titre: 
Transcriptome Analysis from High-Throughput Sequencing Count Data
Résumé: 

The most common RNA-Seq strategy consists of random shearing, amplification, and high-throughput sequencing, of the RNA fraction. Methods to analyze transcription level variations along the genome from the read count profiles generated by the is global RNA-Seq protocol are needed. We developed statistical approaches to estimate the local transcription levels and to identify transcript borders. The transcriptional landscape reconstruction relies on a state-space model to describe transcription level variations in terms of abrupt shifts and more progressive drifts. A new emission model is introduced to capture not only the read count variance inside a transcript but also its short-range autocorrelation and the fraction of positions with zero-counts. The estimation relies on a Sequential Monte Carlo algorithm, the Particle Gibbs.

Année: 
2015
Organisme intervenant: 
Biologie Computationnelle et Quantitative, UMR 7238 CNRS-UPMC; MaIAGE INRA
Date du jour: 
Lundi, Janvier 19, 2015


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